Fig. 3
Evaluation of apoptosis on the compression area in experimental groups (B, D) with TUNEL labeling. Apoptotic cells are labeled green (arrows) and total cells (nuclei) are labeled blue. PDL and hematopoietic marrow are outlined with a white dashed line on images A–D. Note decreased number of alveolar apoptotic cells in orthodontically loaded maxillae treated with IG9402 + OTM24-h (D) compared to the saline + OTM24-h-treated group (B). However, IG9402 + OTM24-h has increased apoptosis in the compressed PDL (D). E Graph showing the comparison of apoptotic cells (TUNEL +) in the AB and PDL following OTM. Saline + OTM24-h has a statistically significant increase in apoptosis in the alveolar bone compared to the IG9402 group (Mann-Whitney p<0.0001). However, IG9402 + OTM24-h has a statistically significant increase in apoptotic cells in the PDL (unpaired t-test, p=0.0196). Each value represents the mean ±SD (n=6)