Table 5 Result characteristics
Ref no. | Mediators | Stats analy appld | Confd | Drop outs | Up/down reg | Pk | Sec outcm | r | Stat sign readings |
|---|---|---|---|---|---|---|---|---|---|
22. | IL-1β | ANOVA and Friedman and paired t | Y | NM | Inc | 24 h, 2 m | 1. Mean tot prot conc - 12 mg/ml | C mov with less pain and inflam with 50 g than with 150 g of F | Inc at 24 h and 2 m in 150 g F compd to cont |
2. VAS score of 150 g > 50 g at 24 h | |||||||||
23. | Leptin | Wilcoxon, Friedman | NM | NM | Dec | 168Â h | NM | NM | b/w bas and 168Â h in exp T |
24 | IL-1β, IL-1RA | ANCOVA | Y | Y | Fluct | NM | For same stress and grw status, max diff in speed were 4.2:1 for 13 kPa in growers and 4.8:1 for 26 kPa in Nn-growers | Higher speeds of T move sign assoc with gen type 2 at IL-1β (+3,954), higher AI, and lower IL-1RA in GCF | NM |
25 | OPG | Shapiro Wilk’s Normality, Wilcoxon, Friedman, Z | NM | NM | Dec | NM | NM | NM | Dec at 1 h, 24 h, 168 h, 1 m, 3 m compd with bas |
26 | IL-1β, βG | 1-tailed paired Student t | Y | NM | Inc | M-010 PM-07 CI-08 | βG sign inc for M - 07 to 010 PM-07,08,010 CI - 06, 07, 010 and dec at O2 for M, PM, CI | Stronger F cause higher levels of both IL-1β and βG | IL-1β sign inc for M - O5 to O10 for PM-O6 to 010. For CI - 04, 06, 07, 09, 010 and dec at O2 for M, PM, CI |
27 | IL-1β, IL-6, IL-8, TNF-α | Mann-Whitney, Kruskal-Wallis | Y | NM | Inc | SD - 24 h LD - T1 | NM | NM | In SD IL-1β, IL-8, TNF-α inc, In LD inc of IL-8 at T1 |
28 | TNF-α | Intergrp - Mann-Whitney U Intragrp - Wilcoxon signed rank | NM | NM | Inc | 24 h | PI, PD, BOP sign higher in HG GCF vol at 1 h and 24 h in the RDG > HG | NM | HG - a stat sign dec at 1 wk compd to 24 h. RDG - inc at 1 h stat sign compd to initial value. Inc in RDG at 1 h > HG |
29 | IL-1β,-TNF-α | 1-way paired t, Mann-Whitney U | NM | NM | Inc | 7 d, 21 d | GCF vol inc on 7 d and 21 d of level and retr | NM | TNF –α diff b/w 3 m (level) and 6 m (bf retr) |
30 | RANKL, OPG | 3-way analysis of variance Tukey’s honest sign diff | Y | NM | RANKL- Inc OPG- Dec | 24 h | avg amt of TM for J > A after 168 h mean vol of GCF in A sign lower than J | GCF vol correl with inflam state | RANKL at 24 h - sign inc levels both in J & A. RANKL and OPG in A < J OPG at 24 h sign dec levels both in J & A. RANKL/OPG for exp T sign > cont after 24 h RANKL/OPG in A < J |
31 | IL-2, 6, 8 | 1-way paired t Mann-Whitney U | NM | NM | IL-2-inc | IL-2 - 7 d, 21 d of level IL-8 - 7 d of level and 7 d, 21 d of retr | GCF vol greater on 7 d and 21 d of level and retr | NM | IL-8 stat sign dec on 7 d of level |
IL-6-N change | |||||||||
IL-8-dec | |||||||||
32 | RANKL, OPG | Mann-Whitney U | NM | NM | RANKL - inc OPG - dec | 24 h | In vitro compres F for 3, 6, 9, 12, 24, 48 h, RANKL was sign inc in stress (+) grp | N sign diff in mean vol of GCF at 24 h b/w exp T and cont T | Mean RANKL values after 24 h in Exp > cont-mean OPG values after 24 h in Exp < cont |
33 | IL-1β, SP, PGE2 | Paired t multiple linear regression analysis | Y | NM | Inc | 1 d | VAS inc sign 1 h and 24 h after insert of sepr and returned to bas after 7 d SP and PGE2 sign higher at 1 d and 7 d | NM | IL-1β of exp > cont at 1 h, 1 d, 7 d |
34 | SP, IL-1β | Mann-Whitney U Spearman’s signed rank | NM | NM | Inc | NM | Avg amt of TM was 1.5 ± 0.4 mm over 168 h-N sign diff in tot prot level at any of exp time periods mean SP levels inc after 8, 24, and 72 h in Exp | N sign diff in mean GCF vol b/w exp and cont T | mean IL-1β levels inc after 8, 24, and 72 h in Exp |
35 | IL-1β, IL-1RA | ANCOVA | Y | NM | NM | 3 d | Inc lag phase with Mx C moved by 4, 13, and 26 kPa. By 52 kPa, distinct lag phase at 3 d, 14 d | Vt vs avg AI in GCF from D sites of Mx C showed a + ve relationship (R2 = 0.44) | mean AI for C moved with 52 kPa sign > all other mean AIs |
36 | IL-8 | Mann-Whitney U Wilcoxon | NM | NM | Inc | 6 d at tension site, and 1st 24Â h at pressure site | GCF vol greater at tension and pressure sites at 24Â h and 30 d | NM | IL-8 at both sites inc at 1 h of F. B/w 24 |
hand 6 d, inc at tension site. IL-8 inc | |||||||||
among grps b/w 0 & 1 h stats sign | |||||||||
37 | IL-1β, PGE2 | Intra-grp: ANOVA Intergrp: 1-way ANOVA | NM | NM | Inc | 24 h | PGE2 inc at 24 h > BS in CF and IF. PGE2 inc in CF and IF at 24 h > cont In CF,PGE2 > cont at 168 h, then fall. In IF, PGE2 remain high for a wk. | NM | IL-1β inc at 24 h > BS, max at 24 h after 1st act in IF. |
In IF, IL-1β inc 24 h after 1st reactivn > cont | |||||||||
In CF and IF, IL-1β inc at 24 h > cont | |||||||||
38 | PGE-2, IL-6, GMCSF | Paired t (intragrp) independent t (J and A) | NM | NM | Inc | 24 h | Median GCF vol. in J > A. In J and A PGE2 inc at 24 h > BS | NM | InJ, IL-6, GMCSF inc at 24 h > BS |
39 | IL-1β, βG | 1-tailed paired Student t | Y | NM | Inc | IL-1β - 81 d βG - 46 d | βG - significantly dec 14 d after prophy. Exp > cont at 46 d | NM | IL-1β significantly dec 14 d after prophy. Exp > cont at 4 d, 33 to 81 d |
40 | IL-1β, IL-1RA, AI | Least square regression Pearson product-moment Correl coefficient | NM | NM | Inc | 3 d | C retr at 1.27 and 0.87 mm/m for 13 and 4 kPa of stress, resp | +ve corr of velocity and AI from D > M. | IL-1β at M > Cont (13 kPa) IL-1RA at D > M and Cont (4 and 13 kPa) Tot prot at M and D > Cont (4 and 13 kPa) |
41 | TGF-β | Student’s t | Y | NM | Inc | 24 h | C retr was 1.1 at 0.1 mm/168 h | NM | TGF-β at exp site at 24 h > bas, Cont |
42 | IL-1β, IL-6, TNF-α, EGF, β2-μG | Student’s t | Y | NM | Inc | 24 h | β-2 MG inc at 24 h > BS or 1 h | NM | Intra-grp in Exp: IL-1β inc at 24 h > bas, IL-6 inc at 24 h > bas or 168 h, TNF-α inc at 24 h > bas or 168 h, EGF inc at 24 h > bas |
β-2 MG in exp at 24 h > ant cont |  | Intergrp btw cont and exp: IL-1β inc in exp > cont at 24 h, mean IL-6 in exp > ant cont, TNF-α in exp at 24 h > ant cont, EGF in exp at 24 h > ant cont | |||||||
19 | IL-1β, PGE | 2-way analy of variance paired t | NM | NM | Inc | 24 h | PGE inc in exp > cont at 24 h, 48 h | NM | IL-1β inc in exp > cont at 1 h, 24 h |
PGE inc at 24 h > BS, 168 h |  | IL-1β inc at 24 h > bas, 48 h, 168 h | |||||||
43 | IL-1β, TNF-α, NO | Friedman, Wilcoxon test Spearman Rank Correl Analy. | Y | NM | Inc | 6 m | PI, GI, PD inc at 1 m > BS, 6 m > BS | NM | IL-1β inc at 6 m > bas |
44 | RANKL, OPG | Repeated measures ANOVA | NM | NM | Inc | 48 h | PM retr −3.73 ± 1.08 mm (laser grp) and 2.71 ± 0.9 mm (Cont grp) Max mean retr v(t) btw 0 and 48 h. Pain intensity pk at 48 h. | NM | RANKL - inc at 48 h > bas |
RANKL/OPG - inc at 48 h > bas | |||||||||
45 | RANKL, HSP70, | ANOVA post hoc Fisher’s LSD | Y | NM | Inc | 24 h | Amylase activity in saliva inc at 24 h at exp > cont | NM | RANKL inc in 24 h > cont |
46 | MMP-8, IL-1β | Paired-sample t | NM | NM | Inc | 3 m | NM | MMP-8 dec at 24 h, inc at 3 m | IL-1β inc at 3 m |
47 | GM-CSF, IF-γ, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-8, IL-10 and TNFα, MMP-9, TIMP-1 and 2, RANKL, OPG | Paired non-parametric Kruskall-Wallis. Spearman Rank Sum analy. | Y | NM | Inc | 4 h | +ve corr of GCF vol and plaque index at 0 at(t), (comp) | +ve corr of TNF-α, IL-1β, IL-8, GM-CSF MMP-9 and TIMP levels to speed of OTM at 4 h in Exp corr of IL-1β, IL-8, TNF-α inc to plaque-induced inflam at 0 at (comp). | Exp - IL-1β, IL-8, TNFα inc from 4 h to 42 d. RANKL - inc after 42 d |
Exp - TIMP 1 and 2 inc at 4Â h, 7 d and 42 d, MMP-9 inc at 4Â h, 7 d. | |||||||||
Compr-MMP −9 inc at 4 h and 7 d. TIMP-1 inc at 4 h and TIMP-2 at 7 d. | |||||||||
Exp > cont: MMP-9 inc at 0, 4 h at compr, TIMP-2 inc at 0 at Exp, compr | |||||||||
48 | RANK, OPG, OPN, TGF-β1 | Friedman Dunn’s multiple comparisons as post hoc | Y | NM | Inc | 7 d | NM | NM | RANK - inc in 7 d in exp, compres > cont OPG - cont > compres site at 24 h. TGF-β - inc in compres > cont at 7 d. |
49 | MMP-3, MMP-9, MMP-13, MIP-1β, MCP-1, RANTES | Friedman, Mann-Whitney | Y | NM | NM | NM | MMPs inc at 1 h, dec at 24 h. GCF vol at (comp) > (t) at 21 d | NM | NM |
50 | IL-1β, SP, PGE2 | SPSS 13.0 paired t-test Wilcoxon paired signed rank | NM | NM | Inc | 1 d | Exp > Cont: | NM | Exp > Cont: |
At D, SP inc at 1 d, 7 d | At M, IL-1β inc at 1 min, 1 h, 1 d, 7 d. | ||||||||
At D, PGE2 inc at 1 min, 1 h, 1 d, 7 d. | At D, IL-1β at 1 h, 1 d, 7 d. | ||||||||
At M, PGE2, SP, inc at 1 min, 1 h, 1 d, 7 d > BS. | Exp > bas: At M, IL-1β inc at 1 min, 1 h, 1 d, 7 d. At D, IL-1β inc | ||||||||
At D, SP, PGE2 inc at 1 d, 7 d > BS. | at 1 h, 1 d, 7 d. | ||||||||
51 | IL-1β | Wilcoxon signed-rank Mann-Whitney U | NM | NM | Inc | 24 h | IL-1β at 24 h, 48 h at exp > implant | NM | IL-1β inc at 24, 48 h > bas IL-1β dec after 24 h, at 48 h, 168 h, 14 d, 21 d. |
52 | IL-2, IL-6, IL-8 | 1-way ANOVA ( interG) Dunnett’s t Tukey’s | NM | NM | Inc | 24 h | IL-8 inc at 24 h, 48 h in MS grp | NM | IL-2 inc at 24 h > bas |
IL-6 inc at 3Â m in MS grp | Â | IL-8 inc in Exp at 1Â h, 24Â h, 48Â h | |||||||
53 | CCL-2 (MCP1), CCL-3, CCL-5 (RANTES), IL-8 (CXCL8), IL-1α, IL-1β, IL-6, TNF-α | ANOVA Tukey’s post hoc paired and unpaired t | Y | NM | Inc | 24 h | MOPs inc the rate of C retr by 2.3-fold compd to cont and contr C - VAS sign at 24 h for Exp and Con | MOPs sign inc cyt and chemokine expression | All cyt and chemo inc in both Cont and Exp at 24 h IL-1 inc also at 28 d |
54 | TNF-α | Paired t (intra G) 1-way ANOVA (interG) Dunnett’s t Tukey’s | NM | NM | Inc | 24 h | NM | NM | TNF-α inc at 24 h |
55 | TNF-α, IL-1β, IL-8 | 1-way ANOVA Paired student t | NM | NM | TNF-α, IL-1β, IL-8 inc in 1 to 2 d of level | 1 d | GCF vol inc in 1 to 3 d of leveling | NM | TNF-α, IL-1β, IL-8 inc in 1 to 2 d of level |
56 | IL-1β, IL-1RA, AI | Least square Regression Pearson product-moment Correl coefficient | NM | NM | AI = 1, then v(t) is not zero | NM | C retr at day 84 for 13, 26, 52 kPa were 4.14 ± 0.19, 6.36 ± 1.32, 5.66 ± 1.38 mm resp | v(t) affected by AI in GCF, stress and IL-1 gene cluster | Faster v(t) seen in 26 kPa, higher GCF and allele 1 homozygosity |
57 | RANKL, OPG, IL-1, IL-1RA, MMP-9 | Repeated measures models | Y | NM | IL-1/IL-1 + IL-1RA - dec RANKL/RANKL + OPG - inc | A - 6 wk | NM | NM | IL-1RA in adults exp > cont at 3 wk. Dec in ratio of (IL-1/[IL-1 + IL-1RA]). Inc in ratio of RANKL to OPG (RANKL/[RANKL + OPG]). RANKL to OPG inc at 6 wk in ado, at 3 wk in A, OPG in ado at Exp < cont at 6 wk |
Ado - 3 wk | |||||||||
58 | IL-2, IL-4, IL-6, IL-8, IL-10, GM-CSF, IFN-γ, TNF-α, MCP-1, IP-10 | Stepwise regression | NM | NM | NM | NM | NM | IL-6 levels at bas predictive of GCF flows after 1 y of ortho t/t | No sign change |
59 | OPG, RANKL | 2-way ANOVA. A Bonferroni f | NM | NM | OPG inc in M, dec in D at 1Â h | 1Â h | NM | NM | OPG inc on M, dec in D at 1Â h |