Effect of low level laser and low intensity pulsed ultrasound therapy on bone remodeling during orthodontic tooth movement in rats

Progress in Orthodontics

Table 1 The primer sequences, melting temperature, their respective product size, and gene bank accession number

Target Gene	Primers	Melting T_m (°C)	Product size	Gene bank accession no.
RANKL	Forward:5′-acgcagatttgcaggactcgac-3′	59.5	493 bp	AF019048
RANKL	Reverse:5′-ttcgtgctccctcctttcatc-3′	57.6
RANK	Forward:5′-ttaagccagtgcttcacggg-3′	57.4	497 bp	AF018253
RANK	Reverse:5′-acgtagaccacgatgatgtcgc-3′	59.5
OPG	Forward:5′-tggcacacgagtgatgaatgcg-3′	59.5	537 bp	U94330
OPG	Reverse:5′-gctggaaagtttgctcttgcg-3′	57.6
RUNX2	Forward:5′-gaaccaagaaggcacagaca-3′	55.4	452 bp	NM053470.2
RUNX2	Reverse:5′-tccaccaccctgttgctgta-3′	55.4
GAPDH	Forward:5′-accacagtccatgccatcac-3′	57.4	452 bp	NM017008
GAPDH	Reverse:5′-tccaccaccctgttgctgta-3′	57.4