Table 1 Growth conditions of each bacterial species for the multi-species biofilm model
From: Orthodontic bonding procedures significantly influence biofilm composition
Bacterial species | Growth condition |
|---|---|
Streptococcus mutans, Streptococcus sobrinus, Streptococcus sanguinis, Streptococcus salivarius, Streptococcus oralis, Actinomyces naeslundii, Lactobacillus rhamnosus, Veillonella dispar, Neisseria subflava | Brain heart infusion medium at 37 °C with 5% CO2 |
Fusobacterium nucleatum, Prevotella nigrescens, Porphyromonas gingivalis | Anaerobic condition with tryptic soy agar medium supplemented with 10 μg/mL vitamin K, 5 μg/mL hemin, and 5% sheep blood at 37 °C for 7 days Subcultured in BHI medium with 10 μg/mL vitamin K and 5 μm/mL hemin and grown to mid-exponential phase anaerobically at 37 °C |
Actinobacillus actinomycetemcomitans | Brain heart infusion medium at 37 °C in an anaerobic condition |