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Table 1 Growth conditions of each bacterial species for the multi-species biofilm model

From: Orthodontic bonding procedures significantly influence biofilm composition

Bacterial speciesGrowth condition
Streptococcus mutans, Streptococcus sobrinus, Streptococcus sanguinis, Streptococcus salivarius, Streptococcus oralis, Actinomyces naeslundii, Lactobacillus rhamnosus, Veillonella dispar, Neisseria subflavaBrain heart infusion medium at 37 °C with 5% CO2
Fusobacterium nucleatum, Prevotella nigrescens, Porphyromonas gingivalisAnaerobic condition with tryptic soy agar medium supplemented with 10 μg/mL vitamin K, 5 μg/mL hemin, and 5% sheep blood at 37 °C for 7 days
Subcultured in BHI medium with 10 μg/mL vitamin K and 5 μm/mL hemin and grown to mid-exponential phase anaerobically at 37 °C
Actinobacillus actinomycetemcomitansBrain heart infusion medium at 37 °C in an anaerobic condition
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