Fig. 2
Micro-CT analysis. A Region of interest (ROI) for alveolar bone analysis (BVF) extended from the mesial side of the distobuccal root to the distal side of the mesiobuccal root of the right maxillary molars (traced area). B Linear measurements of tooth movement were obtained from the intermolar distances (HOC-HOC) on the MicroCT 2D image slice of hemisected maxilla. C Graph showing the comparison of alveolar bone densities (BVF) between experimental groups. BVF bone volume fraction (bone volume divided by total volume). Note the significant decrease in BVF by saline + OTM10-day-treated groups (unpaired t-test, p<0.02). OTM caused no significant difference in BVF in the IG9402 + OTM10-day-treated group (unpaired t-test, p=0.25). Each value represents the mean ±SD (n=4-6). D Graph showing the intermolar distances (HOC-HOC) measured for IG9402 + OTM10-day- and saline + OTM10-day-treated groups. Each value represents the mean± SD (n=4-6). No significant difference was detected in OTM between IG9402-treated and saline-treated groups (unpaired t-test, p=0.6650)