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Table 4 GCF characteristics

From: Effect of orthodontic forces on cytokine and receptor levels in gingival crevicular fluid: a systematic review

Ref no. Time Temp Humd Site sp Inser (in mm) Rep meas I/O meas Meth/O coll Du/O meas Temp of sto Meth/O meas Anal meth Prot conc
22 NM NM NM D 1 mm 2 90 s PP 30 s −80°C PT8000 ELISA pg/mg
23 Y NM NM D 1 mm 4 60 s PP 30 s −80°C PT8000 ELISA pg/ml
24 NM NM NM D NM 2 NM NM NM NM NM ELISA NM
25 NM NM NM D 1 mm N NA PP 30 s −80°C PT8000 ELISA pg/μl
26 NM Y Y M NM N NA PP 30 s NM PT6000 ELISA pg/30 s
27 NM NM NM DB 1 mm N NA PP 30 s −20°C PT8000 MB-IA pg/μl
28 Y NM NM D 1 mm 4 60 s PP 30 s −80°C Elec scale ELISA pg/μl
29 Y NM NM M and D 1 mm N NA PP 30 s −20°C PT8000 IA pg
30 NM NM NM D 1 mm 2 60 s PP 60 s −30°C PT8000 ELISA pg/μl
31 Y NM NM M and D NM N NA PP 30 s −20°C PT8000 IA pg
32 NM NM NM D 1 mm 1 60 s PP 60 s −30°C PT8000 ELISA pg/μl
33 NM NM NM DB and DP 1 mm NM NA DuFM 20 s −70°C NM ELISA pg/20-s samp
34 NM NM NM D 1 mm 2 NM PP 60 s −30°C PT8000 ELISA pg/μg
35 NM NM NM M and D 1 mm 1 60 s PP 30 s −70°C NM ELISA mg/l - tot prot, IL-1-ng/g IL-1RA- μg/g
36 NM NM NM M and D 1 mm 1 60 s PP 30 s −70°C Elect scale ELISA pg/ml
37 Y Y Y D 1 mm 4 1 min PP 30 s −70°C NM ELISA pg/μg
38 NM Y NM MB and DB 1 mm N N PP 30 s −80°C PT6000 RIA pg/μl
39 NM Y Y M NM N NA PP 30 s NM PT6000 ELISA pg/30-s GCF
40 NM NM NM M and D 1 mm 1 1 min PP 30 s −70°C NM ELISA Tot prot - mg/l, IL-1β (ng/g), IL-1RA (μg/g)
41 NM NM NM D 1 mm 1 1 min PP 30 s −30°C PT ELISA, EP, WB pg/μg
42 NM NM NM D 1 mm 1 1 min PP 30 s −30°C PT ELISA pg/μg
19 Y Y y MB 1 mm N N PP 30 s −80°C PT6000 RIA pg
43 NM NM NM M and D NM NM NM PP 30 s NM PT8000 ELISA NM
44 NM NM NM D NM N N PP 30 s −80°C PT8000 ELISA pg/μl
45 NM NM NM D 2 mm 1 2 min PP 1 min −20°C NM ELISA, WB, SDS-PAGE NM
46 NM NM NM M NM N N FP 3 min −30°C NM ELISA IL-1β; pg/ml, MMP-8: ng/ml
47 NM NM NM MB and DP NM N N PP 30 s NM PT8000 LMAT pg/ml
48 NM NM NM ExpT: MB and DB Cont T: MB and ML 1 mm N N PP 30 s NM PT8000 ELISA Cyt conc - pg/μl Tot prot (pg)
49 NM NM NM M and D 1 mm N N PP NM −80°C PT8000 MB-IA pg/site
50 NM NM NM MB and DB 1 mm N N FM 20 s −70°C NM ELISA pg/20 s
51 Y Y Y DB NM N N FP 3 min −70°C NM ELISA pg/μl
52 Y NM NM DB NM 1 1 min PP 30 s −20°C PT8000 ELISA pg/μl
53 Y NM NM DB 1 mm 0 NA FP 10 s NM PT8000 CPA pg/μl
54 Y NM NM DB NM 1 1 min PP 30 s −20°C PT8000 IA pg/μl
55 Y NM NM NM 1 mm N N PP 30 s −20°C PT8000 IA pg
56 NM NM NM D 1 mm 1 1 min PP 30 s −70°C NM ELISA Tot prot - mg/l, IL-1β (ng/g), IL-1RA (μg/g)
57 NM NM NM DL 1 mm N N PP 30 s −80°C NM QAK pg/ml
58 NM NM NM MB and DB NM 2 NA PP 30 s −70°C PT6000 BHCA pg/ml
59 NM NM NM M and D 1 mm 1 1 min PP 30 s −70°C Precisa 62 A ELISA pmol/l
  1. Ref No. reference number, Humd humidity, sp specification, Inser insertion, MB mesio-buccal, ML mesio-lingual, DP disto-palatal sites, DB disto-buccal sites, M mesial, D distal, NM not mentioned, N no, Y yes, PP periopaper, PT periotron, FP filter paper strips, FM Durapore filter membrane, WB Western blot, ELISA enzyme linked immune sorbent assay, SDS-PAGE sodium-dodecyl sulfate polyacrylamide gel electrophoresis, RIA radio-IA, I/O interval of, Meth/O method of, coll collection, meas measurement, Du/O duration of, Temp temperature, sto storage, Anal analysis, Prot protein, conc concentration, pg picogram, mg microgram, ml milliliter, μl microliter, GCF gingival crevicular fluid, tot total, g gram, ng nanogram, s seconds, min minutes, °C degree Celsius, elect electronic, IA immunoassay, EP electrophoresis, sP spectrophotometry, Ar array, A assay, MB multiplex bead, LMAT Luminex multianalyte technology, BHCA Bio-Plex human cytokine assay, CPA custom protein array, QAK Quantibody Ar kit, DuFM Durapore filter membrane.