Table 2 Polymerase chain reaction conditions with respect to bacterial species

Primers for Streptococcus mutans,

Universal primers

Initial denaturation for 30 s at 94 °C

Forty cycles of denaturation for 20 s at 95 °C

Annealing for 45 s at 60 °C

Extension for 10 s at 60 °C

Primers for Porphyromonas gingivalis

Initial denaturation for 1 min at 95 °C

Forty cycles of denaturation for 5 s at 95 °C

Annealing for 15 s at 61 °C

Extension for 33 s at 72 °C

Final extension for 10 min at 72 °C