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Table 2 Polymerase chain reaction conditions with respect to bacterial species

From: Orthodontic bonding procedures significantly influence biofilm composition

Bacterial primerCycling condition
Primers for Streptococcus mutans,
Universal primers
Initial denaturation for 30 s at 94 °C
Forty cycles of denaturation for 20 s at 95 °C
Annealing for 45 s at 60 °C
Extension for 10 s at 60 °C
Primers for Porphyromonas gingivalisInitial denaturation for 1 min at 95 °C
Forty cycles of denaturation for 5 s at 95 °C
Annealing for 15 s at 61 °C
Extension for 33 s at 72 °C
Final extension for 10 min at 72 °C