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Progress in Orthodontics
Fig. 5 | Progress in Orthodontics

Fig. 5

From: Extracellular vesicles derived from M1 macrophages enhance rat midpalatal suture expansion by promoting initial bone turnover and inflammation

Fig. 5

Immunohistochemical staining of midpalatal suture of different groups. A Hematoxylin and eosin staining (H&E staining) of midpalatal sutures after expansion; B Identification of osteoclasts (TRAP staining); C Expression of RANKL; D Expression of p 65; E Expression of BMP-2. F Tartrate-resistant acid phosphatase (TRAP)-positive count; G–I Semiquantitative analysis of RANKL, P 65 and BMP-2; Data were presented as mean ± SD. Differences were analyzed by one-way ANOVA (F–I). Black arrows point to the TRAP-positive osteoclast (B), RANKL positive tissue (C), p 65 positive tissue (D) and BMP-2 positive tissue (E). The E + M1 group displayed significantly higher levels of RANKL and P65 protein expression than any other group and the osteoclast counts were significantly greater. The E + M2 group demonstrated an increase in BMP-2 expression (* P < 0.05, ** P < 0.01, *** P < 0.001). Scale bar: 100 μm

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