Effects of laser-assisted cosmetic smile lift gingivectomy on postoperative bleeding and pain in fixed orthodontic patients: a controlled clinical trial
© Sobouti et al.; licensee Springer. 2014
Published: 9 December 2014
Background and objective
Diode lasers are becoming popular in gingival treatment following orthodontic treatments. Despite their merit and clinical implications, postoperative pain and bleeding after surgery with diode lasers are not assessed except in few controversial studies.
This controlled clinical trial was conducted on 30 healthy orthodontic patients aged 17–29 years, needing esthetic-only gingivectomy in the anterior maxilla. The patients were randomly divided into two groups of 15 each: experimental (laser-assisted surgery) and control (traditional surgery using scalpels). The bleeding rate following the surgery was assessed using the bleeding criteria established by the World Health Organization. The postsurgical pain level was recorded using visual analog scales immediately after the surgery and in patients who consumed analgesics, also 2 h after the analgesic consumption. The data were analyzed using the independent-samples t, Mann-Whitney U, and chi-square tests (α = 0.05).
The average bleeding rates were 1.15 and 0.36 in the conventional and laser groups, respectively (Mann-Whitney U P < 0.05). Experimental patients had no postsurgical pain (VAS1 and VAS2 = 0). In the control group, the average VAS1 pain was 5.2 out of 10. The difference between VAS1 values in the control/experimental groups was significant (Mann-Whitney U P < 0.001).
940-nm diode laser seems promising in reducing postoperative bleeding and pain of patients needing cosmetic smile lift surgeries.
With an ever-increasing number of adults seeking orthodontic treatment, the improvement of patients’ esthetics has become one of the main goals of orthodontics -. The gingival esthetics plays a major role in this regard . Disproportionate dentogingival relationships might negatively affect the outcome of treatment, even if the teeth are perfectly aligned ,.
Orthodontic treatment might affect gingival health -. In certain cases, the gingival margin needs recontouring by means of gingivectomy . However, the costs and postsurgical pain of this treatment might discourage patients, unless in severe cases ,. Pain is one of the most important and common postoperative complications, which can discourage patients from seeking treatment; and its proper control might leave a good impression on the patient regarding the quality of surgery -.
Lasers have been useful in various fields, including orthodontics . With the introduction of soft tissue diode lasers, which might be economic and less painful than conventional methods, the gingivectomy treatment became a routine part of orthodontic treatment. Diode lasers might provide proper hemostasis, reduce the infection risk, and prevent damage to the teeth and bone because of their effect range which is limited to soft tissue . They also might improve esthetics while improving soft tissue healing ,,. Edema, less swelling, and faster healing are the advantages of laser usage in soft tissue management ,.
Gingivectomy can be performed by different means such as scalpels, electrosurgery, chemosurgery, and laser . The conventional surgery performed by a small scalpel has been considered the most common method -. However, the advent of diode lasers highly absorbable by melanin and hemoglobin allows soft-tissue manipulations such as gingival recontouring, operculectomy, or frenectomy accompanied by improved epithelization and wound healing ,,. Lasers can incise the soft tissue to a depth of 2 to 6 mm . The localized heat causes coagulation, protein denaturization, drying, vaporization, and carbonization at the site of the energy absorption. This might seal blood vessels and inhibit pain receptors at the incision location ,. Therefore, using diode lasers might be advantageous because of better control, potentially lower pain and inflammation, and improved wound healing ,,,. Despite these potential advantages, there is only one study comparing traditional method of surgery versus diode laser-assisted surgery in orthodontic setups, which did not enroll a uniform sample of surgeries .
In view of the lack of any studies comparing diode laser with scalpel in gingivectomies, this study was conducted. Its objective was to evaluate comparatively the effects of 940-nm diode laser on postoperative bleeding and pain, in orthodontic patients needing cosmetic smile lift gingivectomy. The null hypotheses were that surgery using diode laser versus scalpel would result in a similar level of postoperative bleeding and pain.
This controlled clinical trial was conducted on 30 patients undergoing fixed orthodontic treatment during 2012 to 2014. The sample size was predetermined as similar to the previous studies’ sample sizes ,,. The protocol ethics were approved by the ethics committee of Mazandaran University of Medical Sciences. All patients were aware of their presence in this study, signed the consent form, and could leave at any time. Patients were selected about 1 month before the completion of their orthodontic treatment. The candidate patients were instructed to maintain a proper level of oral hygiene in order to keep their gingivae healthy.
The exclusion criteria comprised non-orthodontic patients, orthodontic patients with poor oral hygiene, orthodontic patients needing ortho-surgical treatment for esthetic purposes, patients having trismus and limited mouth opening, or those with a history of systemic diseases or any kind of disorders that could affect bleeding as well as pain perception directly or through the taken medications.
The inclusion criteria were patients with no/minimum gingival inflammation/pathology but needing esthetic-only gingivectomy (cosmetic smile lift) in bilateral upper incisors and canines after orthodontic treatment. The included patients needed to be skeletally normal and not needing esthetic skeletal surgeries or any underlying bone removals. Their need for esthetic post-treatment gingivectomy would be determined by an orthodontist and a periodontist, based on micro-esthetic criteria for assessing the alveolar bone height.
The orthodontic treatments were performed by an orthodontist at a private clinic in the Sari city. After finishing the treatment, patients who had minimum gingival inflammation were again asked and taught to improve their oral hygiene and plaque control. Two weeks later, they were assessed by the periodontist and orthodontist. They were enrolled in the study if they had no/minimum gingival inflammation. The surgeries were carried out by a periodontist at a private dental center. The patients were randomly divided into two groups of control (15 subjects who received the conventional surgery using scalpels) and experimental (15 subjects who underwent laser gingivectomy). The randomization was done by the orthodontist and periodontist together, according to the order of the approved patients: the approved patients with odd numbers would be sequentially assigned to the conventional group and those with even numbers would be assigned to the laser group.
In the laser group, the local anesthesia was carried out by the topical application of TAC 20 gel (20% lidocaine, 4% articaine, 2% phenylephrine) to the area. Immediately after the beginning of the operation and also during the surgery, patients would be asked about their sensed pain and discomfort. If they sensed any pains, they would receive infiltration injection of 2% lidocaine plus 1:100,000 epinephrine, upon their request until the surgery was performed under complete local anesthesia. In the laser group, no patient asked for extra anesthesia.
In the control (conventional surgery) group, topical TAC 20 gel was applied similar to the laser group. It did not suffice and the patients expressed pain. They received the infiltration injection of 2% lidocaine (1:100,000 adrenaline). The surgeon asked the patient repeatedly regarding any perceived pain or discomfort, in order to make sure that the operation was carried out under absolute anesthetic conditions.
The extent of soft tissue removal in each patient was determined by the periodontist and the orthodontist together. The surgeon used a scalpel (carbon, No. 15 C) in the control group to trim and form the gingival margin. In the laser group, patients were treated for 30 s per tooth by a 940-nm diode laser (diode Epic, BioLase, USA) with a 400-μm fiber at 0.9-W power. During the gingivectomy, the laser tip was held vertically over the gingival margin. By means of a continuous laser beam, the gingival tissue was removed and formed. At the end of the surgery, about a 1-mm gingival sulcus depth remained ,.
After achieving the ideal gingival contour and a proper height of the clinical crown, the surgery region was cleansed with a cotton roll or microbrush soaked in 3% hydrogen peroxide. When needed (determined by the periodontist), the area was sutured with 3-0 stitches.
Postsurgical bleeding was determined in both groups according to the WHO bleeding criteria: (grade 0) no bleeding, (grade 1) bleeding under the skin and petechial class, (grade 2) mild bleeding, (grade 3) gross bleeding, and (grade 4) mortal bleeding or annoying bleeding .
The pain felt by the patients was evaluated using a visual analog scale (VAS) which was later converted to 11 ranked scores (0: no pain, 10: intolerable pain) as the initial VAS (VAS1) ,. The included patients would be provided analgesics upon their request (Gelophen 400 mg, as many doses as wanted). The time to start taking analgesic was recorded for patients. About 2 h after taking the analgesic capsule(s), pain levels were investigated by a second VAS (VAS2), which was similarly converted to 11 ranks.
As an additional finding, the difference between VAS1 and VAS2 was calculated as an index of analgesic drug effect .
Statistical analyses were performed using the independent-samples t-test, chi-square and Mann-Whitney U tests of SPSS 20.0 (IBM, USA). The level of significance was predetermined as 0.05.
Baseline age (year) and gender of the sample
Mean age (year)
95% CI upper limit
95% CI lower limit
No harms were reported other than the pre-specified outcomes of pain-bleeding. None of the experimental patients needed suturing, scalpel incisions, or injecting local anesthesia. Nevertheless, 11 patients in the control group (73.3%) needed suturing. The difference between the frequencies of suturing in both groups was statistically significant according to the chi-square test (P < 0.001).
The average bleeding rate in the control group was 1.15 (out of 4). This was significantly higher (Mann-Whitney U P < 0.05) than the bleeding rate observed in the experimental group (0.36 out of 4).
Infiltration injection of local anesthesia
None of the patients in the laser group requested additional anesthetic doses. However, all the patients in the conventional group asked for extra local anesthesia. The difference between two groups in terms of their request for receiving analgesics was statistically significant (chi-square P < 0.001).
The experimental patients had no postsurgical pain (VAS1 = 0). In the control group, the average pain was 5.2 out of 10. The VAS1 difference was significant (Mann-Whitney U P < 0.001).
Of the control and experimental subjects, 14 and none consumed analgesics, respectively. The 14 control subjects received the painkillers after an average of 82 min. The frequency of analgesic consumption in the two groups was as well significantly different (chi-square P < 0.001). About 2 h after taking the analgesics, the pain felt by the control subjects decreased to 1.2.
Previous studies have compared the laser with conventional surgery ,,. However, there are no studies comparing the 940-nm diode laser with the conventional surgery in gingivectomy patients, only. In the current study, the bleeding rate in the laser group was less than that in the control group. This was consistent with other studies on the decreased bleeding after laser-assisted surgery ,,,. Laser can incise accurately, has a rather deep penetration, can induce coagulation, and is highly absorbed by hemoglobin. All of these factor might contribute to its appropriate hemostasis ,,,,,-.
Better coagulation also provides a dry and isolated environment, which allows a better control and less infection rate. This might be associated with reduced postsurgical pain ,,. The laser-assisted surgery had an astounding effect on pain among our patients. None of the patients treated with laser requested analgesics, while almost all control patients asked to receive painkillers. This was in agreement with other studies that reported a reduced pain level by using lasers ,,. This might be attributed to the less tissue trauma caused by the laser method ,. The lower need for suturing in the laser group might as well imply this.
This clinical trial was limited by some factors. The subjective quality of pain influenced by interindividual and cultural/demographic variations affects its assessment ,,-. Therefore, we used VAS, which is understandable by most patients, has proper sensitivity, and is reliable/reproducible -,,. As another limitation, analgesic consumption is a confounder of pain ,,,. Therefore, we recorded the pain also before the analgesic consumption. Another limitation was the lack of blinding. However, the patients were not aware of the potential effects of laser on bleeding and pain. Thus, their responses were less likely biased by their knowledge of their group allocation. Moreover, it seemed impossible to blind the operator or the patient in such a design. The generalizability of this study might be reduced by the uniform sample of fixed orthodontic patients all needing esthetic-only gingivectomies, as this is not the case in everyday orthodontic practice. However, such a uniform sample was necessary to establish a proper level of internal reliability. Moreover, the results of this laser cannot be necessarily generalized to other types or wavelengths of lasers. Future multicenter trials performed by different surgeons with different levels of experience might favor the generalizability. As well, such studies can be benefited from split-mouth designs.
Within the limitations of this clinical trial, it seems that the use of 940-nm diode laser in gingivectomy surgery of canine-to-canine cosmetic smile lift might reduce postsurgical pain and bleeding compared to the traditional method of surgery using scalpels. Laser-assisted surgery might also reduce the need for suturing and patients’ demand for analgesics.
- Kau CH, Kantarci A, Shaughnessy T, Vachiramon A, Santiwong P, de la Fuente A, Skrenes D, Ma D, Brawn P: Photobiomodulation accelerates orthodontic alignment in the early phase of treatment. Prog Orthod 2013, 14: 30. 10.1186/2196-1042-14-30PubMed CentralView ArticlePubMedGoogle Scholar
- Mampieri G, Giancotti A: Invisalign technique in the treatment of adults with pre-restorative concerns. Prog Orthod 2013, 14: 40. 10.1186/2196-1042-14-40PubMed CentralView ArticlePubMedGoogle Scholar
- Sarver DM: Principles of cosmetic dentistry in orthodontics: Part 1. Shape and proportionality of anterior teeth. Am J Orthod Dentofacial Orthop 2004, 126: 749–753. 10.1016/j.ajodo.2004.07.034View ArticlePubMedGoogle Scholar
- Khosravanifard B, Rakhshan V, Raeesi E: Factors influencing attractiveness of soft tissue profile. Oral Surg Oral Med Oral Pathol Oral Radiol 2013, 115: 29–37. 10.1016/j.oooo.2012.03.021View ArticlePubMedGoogle Scholar
- Rosa M, Olimpo A, Fastuca R, Caprioglio A: Perceptions of dental professionals and laypeople to altered dental esthetics in cases with congenitally missing maxillary lateral incisors. Prog Orthod 2013, 14: 34. 10.1186/2196-1042-14-34PubMed CentralView ArticlePubMedGoogle Scholar
- Sarver DM, Yanosky M: Principles of cosmetic dentistry in orthodontics: Part 3. Laser treatments for tooth eruption and soft tissue problems. Am J Orthod Dentofacial Orthop 2005, 127: 262–264. 10.1016/j.ajodo.2004.07.036View ArticlePubMedGoogle Scholar
- Sarver DM: Use of the 810 nm diode laser: soft tissue management and orthodontic applications of innovative technology. Pract Proced Aesthet Dent 2006, 18: suppl 7-suppl 13.Google Scholar
- Camargo PM, Melnick PR, Camargo LM: Clinical crown lengthening in the esthetic zone. J Calif Dent Assoc 2007, 35: 487–498.PubMedGoogle Scholar
- Lombardo L, Ortan YO, Gorgun O, Panza C, Scuzzo G, Siciliani G: Changes in the oral environment after placement of lingual and labial orthodontic appliances. Prog Orthod 2013, 14: 28. 10.1186/2196-1042-14-28PubMed CentralView ArticlePubMedGoogle Scholar
- Rakhshan H, Rakhshan V. Effects of the initial stage of active fixed orthodontic treatment and sex on dental plaque accumulation: a preliminary prospective cohort study. Saudi Journal for Dental Research. 2014; DOI: 10.1016/j.sjdr.2014.09.001 [ePub ahead of print]. Rakhshan H, Rakhshan V. Effects of the initial stage of active fixed orthodontic treatment and sex on dental plaque accumulation: a preliminary prospective cohort study.Saudi Journal for Dental Research. 2014; DOI: 10.1016/j.sjdr.2014.09.001 [ePub ahead of print].Google Scholar
- Johal A, Katsaros C, Kiliaridis S, Leitao P, Rosa M, Sculean A, Weiland F, Zachrisson B: State of the science on controversial topics: orthodontic therapy and gingival recession (a report of the Angle Society of Europe 2013 meeting). Prog Orthod 2013, 14: 16. 10.1186/2196-1042-14-16PubMed CentralView ArticlePubMedGoogle Scholar
- Foley TF, Sandhu HS, Athanasopoulos C: Esthetic periodontal considerations in orthodontic treatment - the management of excessive gingival display. J Can Dent Assoc 2003, 69: 368–372.PubMedGoogle Scholar
- Parker S: Low-level laser use in dentistry. Br Dent J 2007, 202: 131–138. 10.1038/bdj.2007.75View ArticlePubMedGoogle Scholar
- Seymour RA, Walton JG: Pain control after third molar surgery. Int J Oral Surg 1984, 13: 457–485. 10.1016/S0300-9785(84)80017-4View ArticlePubMedGoogle Scholar
- de Santana-Santos T, De Souza-Santos AA, Martins-Filho PR, da Silva LC, de Oliveira ESED, Gomes AC: Prediction of postoperative facial swelling, pain and trismus following third molar surgery based on preoperative variables. Med Oral Patol Oral Cir Bucal 2013, 18: e65-e70. 10.4317/medoral.18039PubMed CentralView ArticlePubMedGoogle Scholar
- Slade GD, Foy SP, Shugars DA, Phillips C, White RP Jr: The impact of third molar symptoms, pain, and swelling on oral health-related quality of life. J Oral Maxillofac Surg 2004, 62: 1118–1124. 10.1016/j.joms.2003.11.014View ArticlePubMedGoogle Scholar
- Bienstock DA, Dodson TB, Perrott DH, Chuang SK: Prognostic factors affecting the duration of disability after third molar removal. J Oral Maxillofac Surg 2011, 69: 1272–1277. 10.1016/j.joms.2010.06.211View ArticlePubMedGoogle Scholar
- Capuzzi P, Montebugnoli L, Vaccaro MA: Extraction of impacted third molars. A longitudinal prospective study on factors that affect postoperative recovery. Oral Surg Oral Med Oral Pathol 1994, 77: 341–343. 10.1016/0030-4220(94)90194-5View ArticlePubMedGoogle Scholar
- Lago-Mendez L, Diniz-Freitas M, Senra-Rivera C, Gude-Sampedro F, Gandara Rey JM, Garcia-Garcia A: Relationships between surgical difficulty and postoperative pain in lower third molar extractions. J Oral Maxillofac Surg 2007, 65: 979–983. 10.1016/j.joms.2006.06.281View ArticlePubMedGoogle Scholar
- Haraji A, Rakhshan V. Chlorhexidine gel and less difficult surgeries might reduce post-operative pain, controlling for dry socket, infection and analgesic consumption: a split-mouth controlled randomised clinical trial. J Oral Rehabil. 2014; DOI: 10.1111/joor.12230 [ePub ahead of print].Google Scholar
- Nimeri G, Kau CH, Abou-Kheir NS, Corona R: Acceleration of tooth movement during orthodontic treatment - a frontier in orthodontics. Prog Orthod 2013, 14: 42. 10.1186/2196-1042-14-42PubMed CentralView ArticlePubMedGoogle Scholar
- Ozcelik O, Cenk Haytac M, Kunin A, Seydaoglu G: Improved wound healing by low-level laser irradiation after gingivectomy operations: a controlled clinical pilot study. J Clin Periodontol 2008, 35: 250–254. 10.1111/j.1600-051X.2007.01194.xView ArticlePubMedGoogle Scholar
- Newman MG, Takei HH, Klokkevold PR, Carranza FA: Carranza’s clinical periodontology. 10th edition. W.B. Saunders, Philadelphia; 2006.Google Scholar
- Vescovi P, Corcione L, Meleti M, Merigo E, Fornaini C, Manfredi M, Bonanini M, Govoni P, Rocca JP, Nammour S: Nd:YAG laser versus traditional scalpel. A preliminary histological analysis of specimens from the human oral mucosa. Lasers Med Sci 2010, 25: 685–691. 10.1007/s10103-010-0770-4View ArticlePubMedGoogle Scholar
- Fornaini C, Rocca JP, Bertrand MF, Merigo E, Nammour S, Vescovi P: Nd:YAG and diode laser in the surgical management of soft tissues related to orthodontic treatment. Photomed Laser Surg 2007, 25: 381–392. 10.1089/pho.2006.2068View ArticlePubMedGoogle Scholar
- Matthews DC: Seeing the light - the truth about soft tissue lasers and nonsurgical periodontal therapy. J Can Dent Assoc 2010, 76: a30.PubMedGoogle Scholar
- Ize-Iyamu IN, Saheeb BD, Edetanlen BE: Comparing the 810 nm diode laser with conventional surgery in orthodontic soft tissue procedures. Ghana Med J 2013, 47: 107–111.PubMed CentralPubMedGoogle Scholar
- Miyasaki M: Shedding light on the soft tissue laser. Signature 2004, 11: 11–13.Google Scholar
- Pirnat S: Versatility of an 810 nm diode laser in dentistry: an overview. J Laser Health Acad 2007, 4: 1–9.Google Scholar
- Yague-Garcia J, Espana-Tost AJ, Berini-Aytes L, Gay-Escoda C: Treatment of oral mucocele-scalpel versus CO2 laser. Med Oral Patol Oral Cir Bucal 2009, 14: e469-e474.PubMedGoogle Scholar
- English JD, Peltomaki T, Pham-Litschel K: Soft-tissue diode laser surgery in orthodontics. Mosby’s orthodontic review: Elsevier Health Sciences, St. Louis, Missouri; 2009.Google Scholar
- Kravitz ND, Kusnoto B: Soft-tissue lasers in orthodontics: an overview. Am J Orthod Dentofacial Orthop 2008, 133: S110-S114. 10.1016/j.ajodo.2007.01.026View ArticlePubMedGoogle Scholar
- Webert K, Cook RJ, Sigouin CS, Rebulla P, Heddle NM: The risk of bleeding in thrombocytopenic patients with acute myeloid leukemia. Haematologica 2006, 91: 1530–1537.PubMedGoogle Scholar
- Haraji A, Rakhshan V, Khamverdi N, Alishahi HK: Effects of intra-alveolar placement of 0.2% chlorhexidine bioadhesive gel on dry socket incidence and postsurgical pain: a double-blind split-mouth randomized controlled clinical trial. J Orofac Pain 2013, 27: 256–262. 10.11607/jop.1142View ArticlePubMedGoogle Scholar
- Stubinger S, Saldamli B, Jurgens P, Ghazal G, Zeilhofer HF: Soft tissue surgery with the diode laser - theoretical and clinical aspects. Schweiz Monatsschr Zahnmed 2006, 116: 812–820.PubMedGoogle Scholar
- D’Arcangelo C, Di Nardo Di Maio F, Prosperi GD, Conte E, Baldi M, Caputi S: A preliminary study of healing of diode laser versus scalpel incisions in rat oral tissue: a comparison of clinical, histological, and immunohistochemical results. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2007, 103: 764–773. 10.1016/j.tripleo.2006.08.002View ArticlePubMedGoogle Scholar
- Kafas P, Stavrianos C, Jerjes W, Upile T, Vourvachis M, Theodoridis M, Theodoridis M, Stavrianou I: Upper-lip laser frenectomy without infiltrated anaesthesia in a paediatric patient: a case report. Cases J 2009, 2: 7138. 10.1186/1757-1626-2-7138PubMed CentralView ArticlePubMedGoogle Scholar
- Erdinc AM, Dincer B: Perception of pain during orthodontic treatment with fixed appliances. Eur J Orthod 2004, 26: 79–85. 10.1093/ejo/26.1.79View ArticlePubMedGoogle Scholar
- Xiaoting L, Yin T, Yangxi C: Interventions for pain during fixed orthodontic appliance therapy. A systematic review. Angle Orthod 2010, 80: 925–932. 10.2319/010410-10.1View ArticlePubMedGoogle Scholar
- Bergius M, Kiliaridis S, Berggren U: Pain in orthodontics. A review and discussion of the literature. J Orofac Orthop 2000, 61: 125–137. 10.1007/BF01300354View ArticlePubMedGoogle Scholar
- Krishnan V: Orthodontic pain: from causes to management - a review. Eur J Orthod 2007, 29: 170–179. 10.1093/ejo/cjl081View ArticlePubMedGoogle Scholar
- Krukemeyer AM, Arruda AO, Inglehart MR: Pain and orthodontic treatment. Angle Orthod 2009, 79: 1175–1181. 10.2319/121308-632R.1View ArticlePubMedGoogle Scholar
- Bergius M, Berggren U, Kiliaridis S: Experience of pain during an orthodontic procedure. Eur J Oral Sci 2002, 110: 92–98. 10.1034/j.1600-0722.2002.11193.xView ArticlePubMedGoogle Scholar
- Rakhshan H, Rakhshan V. Pain and discomfort perceived during initial stage of active fixed orthodontic treatment. Saudi Dental Journal. 2015. Rakhshan H, Rakhshan V. Pain and discomfort perceived during initial stage of active fixed orthodontic treatment.Saudi Dental Journal. 2015.Google Scholar
- Scott P, Sherriff M, Dibiase AT, Cobourne MT: Perception of discomfort during initial orthodontic tooth alignment using a self-ligating or conventional bracket system: a randomized clinical trial. Eur J Orthod 2008, 30: 227–232. 10.1093/ejo/cjm131View ArticlePubMedGoogle Scholar
- Caso A, Hung L-K, Beirne OR: Prevention of alveolar osteitis with chlorhexidine: a meta-analytic review. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2005, 99: 155–159. 10.1016/j.tripleo.2004.05.009View ArticlePubMedGoogle Scholar
- Grossi GB, Maiorana C, Garramone RA, Borgonovo A, Creminelli L, Santoro F: Assessing postoperative discomfort after third molar surgery: a prospective study. J Oral Maxillofac Surg 2007, 65: 901–917. 10.1016/j.joms.2005.12.046View ArticlePubMedGoogle Scholar
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