- Open Access
A histological and micro-CT investigation in to the effect of NGF and EGF on the periodontal, alveolar bone, root and pulpal healing of replanted molars in a rat model - a pilot study
© Furfaro et al.; licensee Springer. 2014
- Received: 29 July 2013
- Accepted: 22 November 2013
- Published: 6 January 2014
This study aims to investigate, utilising micro-computed tomography (micro-CT) and histology, whether the topical application of nerve growth factor (NGF) and/or epidermal growth factor (EGF) can enhance periodontal, alveolar bone, root and pulpal tissue regeneration while minimising the risk of pulpal necrosis, root resorption and ankylosis of replanted molars in a rat model.
Twelve four-week-old male Sprague-Dawley rats were divided into four groups: sham, collagen, EGF and NGF. The maxillary right first molar was elevated and replanted with or without a collagen membrane impregnated with either the growth factors EGF or NGF, or a saline solution. Four weeks after replantation, the animals were sacrificed and the posterior maxilla was assessed using histological and micro-CT analysis. The maxillary left first molar served as the control for the corresponding right first molar.
Micro-CT analysis revealed a tendency for all replanted molars to have reduced root length, root volume, alveolar bone height and inter-radicular alveolar bone volume. It appears that the use of the collagen membrane had a negative effect while no positive effect was noted with the incorporation of EGF or NGF. Histologically, the incorporation of the collagen membrane was found to negatively affect pulpal, root, periodontal and alveolar bone healing with pulpal inflammation and hard tissue formation, extensive root resorption and alveolar bone fragmentation. The incorporation of EGF and NGF did not improve root, periodontal or alveolar bone healing. However, EGF was found to improve pulp vascularisation while NGF-improved pulpal architecture and cell organisation, although not to the level of the control group.
Results indicate a possible benefit on pulpal vascularisation and pulpal cell organisation following the incorporation of EGF and NGF, respectively, into the alveolar socket of replanted molars in the rat model. No potential benefit of EGF and NGF was detected in periodontal or root healing, while the use of a collagen membrane carrier was found to have a negative effect on the healing response.
- Dental autotransplantation
- Periodontal healing
- Root healing
- Pulpal regeneration
- Nerve growth factor (NGF)
- Epidermal growth factor (EGF)
Tooth autotransplantation is a reliable treatment alternative for missing or damaged teeth. Unlike the traditional prosthodontic options, a transplanted tooth preserves the dentoalveolar ridge, induces the formation of new supporting structures, continues root formation and erupts and maintains occlusal contacts with the opposing teeth while adapting to orofacial growth and development[2–4].
Factors exist, however, that limit the routine use of this technique. These include pulpal necrosis and inflammation, reduced root formation, root resorption and ankylosis[5, 6]. The means of predictably reducing the complications associated with dental autotransplantation while extending the indications and timing boundaries dictated by the biological healing mechanisms of the pulp, root and periodontal tissues are required.
Past approaches included the use of periodontal ligament (PDL) stimulation techniques, connective tissue transplants, membrane barriers and enamel matrix derivative (EMD) proteins[10, 11]. More recent approaches involve the incorporation of naturally occurring growth factors into the dental transplant site with the aim of enhancing periodontal healing, root formation and pulpal regeneration. However, the limited number of studies performed to date utilising growth factors during dental autotransplantation have reported contrasting results.
Results obtained by Komatsu and co-workers suggest that the topical application of platelet-derived growth factor (PDGF) to replanted first molar teeth in the rat effectively promotes restoration of the support function of the healing PDL while minimising the risk of ankylosis. Springer and colleagues found that the incorporation of bone morphogenetic protein-7 (BMP-7) into the tooth socket prior to replantation in minipigs improved the survival rate, but only when the PDL was partially traumatised. When there was minimal or total destruction of the PDL prior to replantation, there was no difference in survival rates amongst the control and BMP-7 groups. In a series of studies performed by Sorensen et al. and Wikesjo et al. the topical application of bone morphogenetic protein-12 (BMP-12) to replanted teeth in Labrador mongrel dogs did not have an apparent effect on new cementum and PDL formation or on the presence and extent of ankylosis when compared to controls.
To date, no studies have looked into the effect of nerve growth factor (NGF) or epidermal growth factor (EGF) on the healing of pulpal, root, alveolar and periodontal tissues subsequent to tooth transplantation.
EGF enhances cellular proliferation and differentiation of epidermal and epithelial cells, fibroblasts, and cartilage and bone derived cells during growth, maturation and healing[16–19]. Following dentoalveolar trauma, it is speculated that circulating EGF is released from the platelets during blood clot formation where it mediates the recruitment of PDL precursor cells and their proliferation[20, 21]. As the PDL precursor cells mature, the role of EGF changes to regulate the differentiation of hard-tissue forming cells and their synthetic activities.
NGF is a target-derived neurotrophic factor essential for the development, growth, survival, differentiation and maintenance of sympathetic and sensory neurones, including those of the dental pulp[22, 23]. Emerging evidence indicates that NGF may have a broader physiological effect than regulating neuronal functions. Studies demonstrate that NGF is involved in bone tissue healing by activating osteoblasts, tubular dentine formation by stimulating preodontoblasts and enhancing the proliferation and differentiation of PDL cells[25–28].
This study aims to investigate, utilising micro-computed tomography (micro-CT) and histology, whether the topical application of NGF and/or EGF can enhance periodontal, alveolar bone, root and pulpal tissue regeneration after autotransplantation while minimising the risk of pulpal necrosis, root resorption and ankylosis.
All experimental procedures administered to the animals were carried out in accordance with the protocol approved by the Animal Care and Veterinary Services Research committee of the University of Western Australia.
Summary of the experimental groups
Number of animals
Molar replantation only - no collagen scaffold or growth factor
Molar replantation, collagen scaffold, no growth factor (saline)
EGF and collagen
Molar replantation, collagen scaffold impregnated with EGF
NGF and collagen
Molar replantation, collagen scaffold impregnated with NGF
All animals were housed at The University of Western Australia (QEII Medical Centre, M Block level 2) animal housing facility in accordance with the guidelines of the NHMRC Code of Practice for the care and use of animals for scientific purposes.
Surgical procedure and tissue preparation
Prior to surgery, all animals were anaesthetised with an intra-peritoneal injection of ketamine hydrochloride (75 mg/kg) mixed with xylazine hydrochloride (10 mg/kg) diluted in sterile saline. To minimise postoperative pain, buprenorphine (0.05 mg/kg) and meloxicam (1 mg/kg) were administered shortly after the anaesthesia via subcutaneous injection.
Depending on the experimental group (Table 1), a 2-mm-square piece of collagen membrane (Koken, Tokyo, Japan) soaked in sterile saline, NGF (R&D Systems, Minneapolis, MN, USA) at concentration of 0.5 mg/ml, or EGF (R&D Systems, Minneapolis, MN, USA) at concentration of 0.5 mg/ml was placed at the base of the socket prior to the replantation of the tooth (Figure 1B). Concentration used was based on a previous study demonstrating a therapeutic effect with EGF. In the sham group, no collagen membrane was placed prior to replantation.
The rats received a soft diet for 1 week before being placed on the standard rat feed pellets for normal occlusal loading. Postoperative analgesics were administered by a subcutaneous injection of buprenorphine (0.05 mg/kg) and meloxicam (1 mg/kg) every 24 h for 3 days.
The animals were observed daily and their body weight were recorded 3 times a week to ensure normal growth and health. The animals were sacrificed 4 weeks after the surgery with methoxyflurane inhalation followed by cardiac injection of a lethal overdose of sodium pentobarbital (150 mg/mL). Four weeks was selected with previous studies which showed that the majority of periodontal and pulpal healing occurred within 2 to 4 weeks after transplantation[31, 32]. A block resection of the maxilla was made and the specimens were fixed in 10% neutral buffered formalin (pH 7.4) overnight at 4°C.
Micro-CT imaging and analysis
For three-dimensional analysis of the teeth and alveolar bone, fixed maxillary samples were scanned at the National Imaging Facility, Centre for Microscopy, Characterisation and Analysis (CMCA), The University of Western Australia, by means of a micro-CT system (SkyScan 1176 in vivo micro-CT, Kontich, Belgium). The specimens were scanned at a resolution of 9 μm, ensuring that all molar teeth and surrounding alveolar bone of the posterior maxilla were encompassed.
Histological preparation and analysis
After micro-CT imaging, the samples were washed in PBS and demineralised in 10% EDTA solution at pH 7.4 for 4 weeks. Before embedding in paraffin wax, the tissues were dehydrated through graded alcohol. Serial sagittal sections of 5 μm were made through the midline of the teeth allowing the mesial root, pulp chamber and inter-radicular alveolar bone to be observed simultaneously. The sections were stained with haematoxylin and eosin (H & E) prior to histological examination.
Descriptive analysis of the teeth focused on the continuation of root formation, the presence or absence of cementum covering the root surface, formation of PDL, presence or absence of root ankylosis and resorption, the quality of the bone surrounding the root and the vitality of the pulp cells.
Formal statistical analyses were carried out using liner mixed models. A linear mixed model approach was taken in each instance with fixed factors group and side and their corresponding interaction. Random effect of individual within treatment was used. Estimated means for left and right percentage difference are provided by group, along with standard errors and p values for these comparisons. All analyses are carried out using R: a language and environment for statistical computing (2012).
Between-group comparison that reveals tissue volume in the inter-radicular region of the upper right first molar was significantly reduced in the collagen and EGF groups compared to the sham group but not so for the NGF group. There was no statistically significant difference in the bone tissue fraction for any of the four experimental groups indicating that bone density did not alter significantly (Figure 8).
Periodontal and root healing
It was hoped that the incorporation of EGF and NGF into the alveolar socket prior to molar replantation in the rat model would aid in periodontal healing. However, no benefit of incorporating these growth factors was observed in this study. Replanted molars in the EGF and NGF groups had similar rates of extensive root resorption as did the collagen only group.
Micro-CT and histological assessment revealed that all replanted rat molars had significantly reduced root lengths and root volumes compared to the untouched left molar. This suggests that the addition of the collagen membrane, with or without EGF and NGF, to the alveolar socket prior to replantation negatively affected root healing and development. It is known that root growth is dependent upon the coordinated activity of Hertwig's epithelial root sheath, the pulp and the PDL cells. Continued root development after transplantation can only be expected if Hertwig's epithelial root sheath is preserved around the apices suggesting that trauma was sustained to the root sheath during transplantation, especially in the experimental groups involving a collagen membrane.
Although all the molar replantations were carried out as atraumatic as possible, some damage to the dentoalveolus inevitably occurred. This was reflected by a general tendency for increased alveolar trabeculisation and fragmentation, and decreased bone and tissue volumes in all the experimental groups. The incorporation of EGF or NGF, when compared to the collagen only group, showed no significant effect on alveolar healing. Additionally, compared to the sham group, the use of a collagen membrane with or without any growth factors seemed to make the dentoalveolar healing worse. It appears that the presence of the relatively rigid collagen membrane may have interfered with the normal alveolar bone healing, and any possible benefit the growth factors may have had. This suggests that the use of collagen membrane as a protein carrier may not be ideal for in vivo studies involving the rat model.
A variety of new injectable materials such as hydrogels are being developed for growth factor delivery applications. These injectable gels are especially attractive because they can allow for minimally invasive delivery of inductive molecules which is beneficial when dealing with delicate structures such as rat alveolar bone and epithelial root sheaths. However, King et al. reported that the more slowly dissolving collagen membrane carrier system allowed for more prolonged exposure to BMP-2 while being able to maintain the growth factor within the required region better than a gel carrier system when assessing wound healing of periodontal fenestration defects in a rat model. Further research is therefore required into the gel carrier systems before they are suitable for use in a clinical setting.
An important factor affecting the survival rate of transplanted teeth is the response of the pulp to the trauma sustained. If pulpal necrosis occurs, there is the possibility of periapical inflammation and inflammatory root resorption, leading to the eventual loss of the transplant[36, 37]. Immature roots consisting of a wide, open apical foramen have improved rates of healing compared to mature teeth[38, 39]. Since the root development of the maxillary first molars from four-week old rats is immaturely developed, the prognosis of pulpal healing should be good. This was observed in the current study with all the replanted molars demonstrating histologically successful pulpal revascularization.
It was interesting to observe that the pulps from the EGF group showed improved vascularisation compared to the collagen only and NGF groups. EGF is known to promote angiogenesis in vivo and EGF receptors have been localised in the dental pulp in the rat. Derringer and Linden have shown that the addition of anti-h EGF to pulp cell culture reduced the angiogenic response with significantly fewer micro-vessel formations. Therefore, we hypothesise that the addition of EGF may have accelerated pulpal revascularisation after dental replantation and induced earlier pulpal healing. Further investigation will be required over multiple time points to assess the actual vascularisation rate and to determine if this enhanced pulpal revascularization will also occur in mature teeth with smaller apical foramina.
Of equal importance was the observation that the pulpal architecture and cell organisation in the NGF group which more closely resembled that of the control group compared to the collagen only and EGF groups. In vitro studies demonstrate that nerve fibres selectively grow only in a local environment containing NGF and show preferential orientation following NGF concentration gradients. The developmental role for NGF is consistent with the early presence of NGF receptor (NGF-R) in the pulp. Upon binding to NGF-R, NGF could exert a wide range of effects on odontogenic cells by providing the positionally and temporally correct microenvironment. This study appears to support the idea that in addition to functions concerning dental neurobiology, NGF may influence the timing, sequence and position for numerous dental cell phenotypes localised in the healing dental pulp.
Possible beneficial effects of incorporating growth factors into the socket of a replanted molar in the rat model include improved pulpal vascularisation with the use of EGF and improved pulp cell organisation with NGF. No beneficial effects were observed in regards to root, alveolar or periodontal healing. In addition, the use of a collagen membrane carrier appeared to negatively affect the healing of the replanted molar.
The authors acknowledge the facilities and the scientific and technical assistance of the National Imaging Facility at the Centre for Microscopy, Characterisation & Analysis, The University of Western Australia, a facility funded by the University, State and Commonwealth Governments. We also thank the Australian Society of Orthodontists Inc. Foundation for Education and Research for their financial support.
- Czochrowska EM, Stenvik A, Bjercke B, Zachrisson BU: Outcome of tooth transplantation: survival and success rates 17–41 years posttreatment. Am J Orthod Dentofacial Orthop 2002, 121(2):110–9. 10.1067/mod.2002.119979View ArticlePubMedGoogle Scholar
- Jonsson T, Sigurdsson TJ: Autotransplantation of premolars to premolar sites. A long-term follow-up study of 40 consecutive patients. Am J Orthod Dentofacial Orthop 2004, 125(6):668–75. 10.1016/j.ajodo.2003.12.002View ArticlePubMedGoogle Scholar
- Bauss O, Engelke W, Fenske C, Schilke R, Schwestka-Polly R: Autotransplantation of immature third molars into edentulous and atrophied jaw sections. Int J Oral Maxillofac Surg 2004, 33(6):558–63. 10.1016/j.ijom.2003.10.008View ArticlePubMedGoogle Scholar
- Zachrisson BU, Stenvik A, Haanæs HR: Management of missing maxillary anterior teeth with emphasis on autotransplantation. Am J Orthod Dentofacial Orthop 2004, 126(3):284–8.View ArticlePubMedGoogle Scholar
- Kallu R, Vinckier F, Politis C, Mwalili S, Willems G: Tooth transplantations: a descriptive retrospective study. Int J Oral Maxillofac Surg 2005, 34: 745–55. 10.1016/j.ijom.2005.03.009View ArticlePubMedGoogle Scholar
- Kim E, Jung JY, Cha IH, Kum KY, Lee SJ: Evaluation of the prognosis and causes of failure in 182 cases of autogenous tooth transplantation. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2005, 100(1):112–9. 10.1016/j.tripleo.2004.09.007View ArticlePubMedGoogle Scholar
- Gault PC, Warocquier-Clerout R: Tooth auto-transplantation with double periodontal ligament stimulation to replace periodontally compromised teeth. J Periodontol 2002, 73(5):575–83. 10.1902/jop.2002.73.5.575View ArticlePubMedGoogle Scholar
- Andreasen JO, Kristerson L: Evaluation of different types of autotransplanted connective tissues as potential periodontal ligament substitutes: an experimental replantation study in monkeys. Int J Oral Surg. 1981, 10: 189–201. 10.1016/S0300-9785(81)80053-1View ArticlePubMedGoogle Scholar
- Gerard E, Membre H, Gaudy J-F, Mahler P, Bravetti P: Functional fixation of autotransplanted tooth germs by using bioresorbable membranes. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2002, 94(6):667–72. 10.1067/moe.2002.128020View ArticlePubMedGoogle Scholar
- Iqbal MK, Bamaas N: Effect of enamel matrix derivative (EMDOGAIN®) upon periodontal healing after replantation of permanent incisors in Beagle dogs. Dent Traumatol 2001, 17(1):36–45. 10.1034/j.1600-9657.2001.170107.xView ArticlePubMedGoogle Scholar
- Ninomiya M, Kamata N, Fujimoto R, Ishimoto T, Suryono KJ-i, Nagayama M, Nagata T: Application of enamel matrix derivative in autotransplantation of an impacted maxillary premolar: a case report. J Periodontol 2002, 73(3):346–51. 10.1902/jop.2002.73.3.346View ArticlePubMedGoogle Scholar
- Komatsu K, Shibata T, Shimada A, Shimoda S, Oida S, Kawasaki K: Biomechanical properties of healing periodontal ligament after replantation of teeth treated with PDGF. J Biomech 2006, 39(1):S566.View ArticleGoogle Scholar
- Springer ING, Acil Y, Spies C, Jepsen S, Warnke PH, Bolte H, Kuchenbecker S, Russo PA, Wiltfang J, Terheyden H: RhBMP-7 improves survival and eruption in a growing tooth avulsion trauma model. Bone 2005, 37(4):570. 10.1016/j.bone.2005.04.037View ArticlePubMedGoogle Scholar
- Sorensen RG, Polimeni G, Kinoshita A, Wozney JM, Wikesjo UME: Effect of recombinant human bone morphogenetic protein-12 (rhBMP-12) on regeneration of periodontal attachment following tooth replantation in dogs - a pilot study. J Clin Periodontol 2004, 31(8):654–61. 10.1111/j.1600-051X.2004.00540.xView ArticlePubMedGoogle Scholar
- Wikesjo UME, Sorensen RG, Kinoshita A, Li XJ, Wozney JM: Periodontal repair in dogs: effect of recombinant human bone morphogenetic protein-12 (rhBMP-12) on regeneration of alveolar bone and periodontal attachment - a pilot study. J Clin Periodontol 2004, 31(8):662–70. 10.1111/j.1600-051X.2004.00541.xView ArticlePubMedGoogle Scholar
- Carpenter G, Cohen S: Epidermal growth factor. J Biol Chem 1990, 265(14):7709–12.PubMedGoogle Scholar
- Cohen S: Epidermal growth factor. Biosci Rep 1986, 6(12):1017–28. 10.1007/BF01141022View ArticlePubMedGoogle Scholar
- Gospodarowicz D, Greenburg G, Bialecki H, Zelter BR: Factors involved in the modulation of cell proliferation in vivo and in vitro: the role of fibroblast and epidermal growth factor in the proliferative response of mammalian cells. In Vitro. 1978, 14: 85–118. 10.1007/BF02618177View ArticlePubMedGoogle Scholar
- Guajardo G, Okamoto Y, Gogen H, Shanfeld JL, Dobeck J, Herring AH, Davidovitch Z: Immunohistochemical localization of epidermal growth factor in cat paradental tissues during tooth movement. Am J Orthod Dentofacial Orthop 2000, 118(2):210–9. 10.1067/mod.2000.104097View ArticlePubMedGoogle Scholar
- Cho MI, Lin WL, Garant PR: Occurrence of epidermal growth factor binding sites during differentiation of cementoblasts and periodontal ligament fibroblasts of the young rats: A light and electronic microscopic radioautographic study. Anat Rec 1991, 231: 14–24. 10.1002/ar.1092310104View ArticlePubMedGoogle Scholar
- Ben-Erza J, Sheiabni K, Hwang DL, Lev-Ran A: Megakaryocyte synthesis is the source of epidermal growth factor in human platelets. Am J Pathol 1990, 137: 755–9.Google Scholar
- Eppley BL, Snyders RV, Winkelmann TM, Roufa DG: Efficacy of nerve growth factor in regeneration of the mandibular nerve: a preliminary report. J Oral Maxillofac Surg 1991, 49(1):61–8. 10.1016/0278-2391(91)90268-QView ArticlePubMedGoogle Scholar
- Kaplan DR, Miller FD: Neurotrophin signal transduction in the nervous system. Curr Opin Neurobiol 2000, 10(3):381–91. 10.1016/S0959-4388(00)00092-1View ArticlePubMedGoogle Scholar
- Mitsiadis TA, Dicou E, Joffre A, Magloire H: Immunohistochemical localization of nerve growth factor (NGF) and NGF receptor (NGF-R) in the developing first molar tooth of the rat. Differentiation 1992, 49(1):47–61. 10.1111/j.1432-0436.1992.tb00768.xView ArticlePubMedGoogle Scholar
- Xu WP, Mizuno N, Shiba H, Takeda K, Hasegawa N, Yoshimatsu S, Inui T, Ozeki Y, Niitani M, Kawaguchi H, Tsuji K, Kato Y, Kurihara H: Promotion of functioning of human periodontal ligament cells and human endothelial cells by nerve growth factor. J Periodontol 2006, 77(5):800–7. 10.1902/jop.2006.050183View ArticlePubMedGoogle Scholar
- Woodnutt DA, Wager-Miller J, O'Neill PC, Bothwell M, Byers MR: Neurotrophin receptors and nerve growth factor are differentially expressed in adjacent nonneuronal cells of normal and injured tooth pulp. Cell Tissue Res 2000, 299(2):225–36. 10.1007/s004410050020View ArticlePubMedGoogle Scholar
- Asaumi K, Nakanishi T, Asahara H, Inoue H, Takigawa M: Expression of neurotrophins and their receptors (TRK) during fracture healing. Bone 2000, 26(6):625–33. 10.1016/S8756-3282(00)00281-7View ArticlePubMedGoogle Scholar
- Byers MR, Schatteman GC, Bothwell M: Multiple functions for NGF receptor in developing, aging and injured rat teeth are suggested by epithelial, mesenchymal and neural immunoreactivity. Development 1990, 109(2):461–71.PubMedGoogle Scholar
- Kvinnsland I, Heyeraas KJ, Byers MR: Regeneration of calcitonin gene-related peptide immunoreactive nerves in replanted rat molars and their supporting tissues. Arch Oral Biol 1991, 36(11):815–26. 10.1016/0003-9969(91)90031-OView ArticlePubMedGoogle Scholar
- Hodde JP, Record RD, Liang HA, Badylak SF: Vascular endothelial growth factor in porcine-derived extracellular matrix. Endothelium 2001, 8(1):11–24.View ArticlePubMedGoogle Scholar
- Tsukiboshi M: Autotransplantation of teeth: requirements for predictable success. Dent Traumatol 2002, 18(4):157–80. 10.1034/j.1600-9657.2002.00118.xView ArticlePubMedGoogle Scholar
- Schwartz O, Andreasen JO: Allo- and autotransplantation of mature teeth in monkeys: a sequential time-related histoquantitative study of periodontal and pulpal healing. Dent Traumatol 2002, 18(5):246–61. 10.1034/j.1600-9657.2002.00085.xView ArticlePubMedGoogle Scholar
- Andreasen JO, Kristerson L, Andreasen FM: Damage of the Hertwig's epithelial root sheath: effect upon root growth after autotransplantation of teeth in monkeys. Dent Traumatol 1988, 4(4):145–51. 10.1111/j.1600-9657.1988.tb00313.xView ArticleGoogle Scholar
- Kaigler D, Cirelli JA, Giannobile WV: Growth factor delivery for oral and periodontal tissue engineering. Expert Opin Drug Deliv 2006, 3(5):647–62. 10.1517/17425247.3.5.647PubMed CentralView ArticlePubMedGoogle Scholar
- King GN, King N, Hughes FJ: Effect of two delivery systems for recombinant human bone morphogenetic protein-2 on periodontal regeneration in vivo. J Periodontal Res 1998, 33(3):226–36.View ArticlePubMedGoogle Scholar
- Claus I, Laureys W, Cornelissen R, Dermaut LR: Histologic analysis of pulpal revascularization of autotransplanted immature teeth after removal of the original pulp tissue. Am J Orthod Dentofacial Orthop 2004, 125(1):93–9. 10.1016/S0889-5406(03)00619-XView ArticlePubMedGoogle Scholar
- Skoglund A, Tronstad L: Pulpal changes in replanted and autotransplanted immature teeth of dogs. J Endod 1981, 7(7):309–16. 10.1016/S0099-2399(81)80097-0View ArticlePubMedGoogle Scholar
- Kristerson L, Andreasen JO: Influence of root development on periodontal and pulpal healing after replantation of incisors in monkeys. Int J Oral Surg 1984, 13(4):313–23. 10.1016/S0300-9785(84)80039-3View ArticlePubMedGoogle Scholar
- Skoglund A, Tronstad L, Wallenius K: A microangiographic study of vascular changes in replanted and autotransplanted teeth of young dogs. Oral Surg Oral Med Oral Pathol 1978, 45(1):17–28. 10.1016/0030-4220(78)90217-7View ArticlePubMedGoogle Scholar
- Schreiber AB, Winkler ME, Derynck R: Transforming growth factor alpha: a more potent angiogenic mediator than epidermal growth factor. Science 1986, 232: 1250–3. 10.1126/science.2422759View ArticlePubMedGoogle Scholar
- Davideau JL, Sahlberg C, Thesleff I, Bendal A: EGF receptor expressed in mineralised tissues; an in situ hybridisation and immunocytochemical investigation in rat and human mandibles. Connect Tissue Res 1995, 32: 43–7.View ArticleGoogle Scholar
- Derringer K, Linden R: Epidermal growth factor released in human dental pulp following orthodontic force. Eur J Orthod 2007, 29: 67–71. 10.1093/ejo/cjl059View ArticlePubMedGoogle Scholar
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